Multispecific Ligands

Affilin® Applications

Multi-Specific Ligands

The modular fashion with which Affilin® ligands can be fused together makes creating multi-specific binders an easy task. Combined with varying linker lengths, composition, and orientation, plus the optional addition of further accessory modules, our Navigo® experts build the designed molecules as previously planned on the drawing board.

Affilin® ligands can be readily fused to the N- or C-terminus of an antibody’s heavy or light chain converting it into a bispecific format (Mabfilin®).

The ease of creating multi-specific binders with Affilin® molecules has far-reaching potential in many fields. Of particular interest is the application of multi-specific ligands for targeting cancer cells, to circumvent problems like tumor escape and antigen loss. Moreover, being small molecules (8,5kDa), multiple Affilin® sequences can be easily packed into a lentiviral system for cell surface expression, offering greater flexibility with the limited genomic space.

Mabfilin®: Affilin®-Antibody Hybrids

A classical antibody can be readily converted to a bi/multi-specific one by fusing it genetically with Affilin® molecules. Synergistically bringing together two target-binding modalities results in a Mabfilin® Affilin®-antibody hybrid that simultaneously addresses several targets and has excellent manufacturability.

Navigo’s proprietary HER2-specific Affilin® fused C-terminally to an EGFR-specific monoclonal antibody (Cetuximab) resulted in a Mabfilin® capable of binding both HER2 and EGFR simultaneously.